Spectroscopy

The Keck Biophysics Facility provides access to several specialized instruments for absorbance, fluorescence, and circular dichroism spectroscopy.

  • UV/Vis/NIR Spectrophotometer

    The Perkin Elmer LAMBDA 1050 is a high performance UV/Vis/NIR double beam, double monochromator, ratio-recording spectrophotometer. A 150 mm integrating sphere accessory allows diffuse reflectance and transmission measurements.

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  • Circular Dichroism Spectrophotometer

    Our state of the art Jasco J-815 CD instrument allows the simultaneous acquisition of fluorescence and CD data. Such experiments are particularly useful in assessing the extent to which protein unfolding and folding transitions are two-state in character.

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  • Fluorescence Plate Reader

    The Molecular Devices Gemini EM Fluorescence/Chemiluminescence Plate Reader complements the facility’s fluorimeters PC1 and K2 by accommodating high throughput fluorescence experiments.

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  • Fluorescence Polarization

    The PanVera (Invitrogen) Beacon 2000 Variable Temperature Fluorescence Polarization System provides a simple, rapid, and highly sensitive way to study equilibrium binding for a range of biomolecules. It measures binding affinities directly in solution and can be used to study protein-protein, protein-DNA, or receptor-ligand binding and interactions.

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  • Photon-Counting Fluorescence Spectrometer

    The ISS PC1 spectrofluorimeter is a workstation for a wide variety of highly sensitive fluorescence studies. The PC1 has excitation/emission monochromators and optional polarizers suitable for fluorescence polarization/anisotropy and ratio measurements using both T- and L- configuration.

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  • Stopped Flow Spectrometer

    The Applied Photophysics SX20 Stopped Flow Spectrometer is a powerful workstation for kinetic studies.

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  • UV/Vis DAD Spectrophotometer

    The HP (Agilent) 8452 diode array spectrophotometer is a basic analytical instrument for measuring UV/Vis spectra of solution samples in seconds.

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  • NanoDrop 3300 Fluorimeter

    The Thermo Scientific NanoDrop 3300 fluorimeter acquires full range visible emission spectra from micro-volume samples in seconds.

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  • NanoDrop Spectrophotometer

    The Thermo Scientific NanoDroop 2000c acquires full range UV/Vis absorbance spectra from micro-volume or cuvette samples in seconds.

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