Surface Plasmon Resonance Spectrometer

Reichert 4- channel surface plasmon resonance spectrometer for label-free measurement of macromolecular association and dissociation. SPR is suitable for a wide range of interactants including proteins, nucleic acids, lipids, carbohydrates, small  drugs and synthetic  polymers and nanoparticles.

http://www.reichertspr.com/systems/spr-systems/four-channel-reichert4spr/

Features:

  • 4 channel channel acquisition
  • Autosampler holding up to 96 HPLC vials or two 96 or 384 well plates
  • Controllable injection volume to conserve precious analytes
  • Optional sampling cooling for extended assays
  • Interaction temperature controllable between 10°C and 70°C
  • Detection of small molecule analytes down to ~100 daltons
  • Accessible range of binding affinities from 1 mM to 1 pM

Immobilization Techniques:

  • Amine coupling to carboxylated planar monolayer or branched dextran sensor chips
  • Thiol coupling using a native or introduced cysteine
  • NeutrAvidin, streptavidin, or avidin capture of biotinylated molecules
  • Nickel NTA capture of histidine-tagged molecules
  • Protein A capture of antibodies
  • Lipid monolayer formation on a hydrophobic surface

Reichert provides an assortment of sensor chips to accommodate different immobilizations. Please see here for a complete list of available sensor chips.

 

NU Core ID: SPR