The SEC-MALS-QELS system is composed of an Agilent 1260 series HPLC for size exclusion chromatography followed by a Wyatt DAWN HELEOS II multi-angle static light scattering detector, a Wyatt QELS dynamic light scattering detector, and a Wyatt T-rEx differential refractive index detector.
Wyatt DAWN HELEOS II – multi-angle (static) light scattering (MALS) detector. The molar mass and root mean square radius of the solute are determined by measuring the intensity of the scattered light – provided that the refractive index increment (dn/dc) of the solute/solvent system is known.
Wyatt Optilab T-rEx – refractometer for measuring the absolute refractive index and its increment
Wyatt QELS – quasi-elastic (dynamic) light scattering (QELS) detector. The diffusion coefficient/hydrodynamic radius can be determined from the temporal fluctuations in the intensity of scattered light
HPLC – with size-exclusion columns (SEC), DAD absorbance, and fluorescence detectors
In this set-up the sample components are separated according to size and characterized individually by static and dynamic light scattering, absorbance and fluorescence.
- For proteins and nucleic acids: measurements of molecular weights and radii, oligomerization, heterogeneity and solubility studies, characterization of macromolecular complexes in terms of size, overall shape and composition/stoichiomentry
- For synthetic polymers: measurements of molar mass & mass moments, polydispersity, conformation/branching
- For nanoparticles: solubility and size distribution measurements and composition /stoichiometry analysis for functionalized nanoparticles
NU Core ID: MALS QELS