BLItz Bio-Layer Interferometer

                   

The BLItz is a micro volume instrument for characterizing the kinetics of macromolecular interactions using bio-layer interferometry with low cost disposable sensor probes. We have an instrument located on each campus: Tarry building (Chicago) and Cook Hall (Evanston).

Overview:

BLItz™ uses ForteBio’s Dip and Read™ label-free assays. These direct binding assays take place on a disposable biosensor made from a biocompatible matrix that is uniform, non-denaturing and minimizes non-specific binding. Only molecules that bind directly to the biosensor surface are detected, providing exceptional specificity for individual applications, even in crude media.

The BLI (bio-layer interferometry) technology used by BLItz provides real-time data on protein interactions. BLItz emits white light down the biosensor, and then collects any light reflected back. Reflected wavelengths are affected by the thickness of the coating on the optical layer. Some wavelengths show constructive interference (blue), others show destructive interference (red).

This interference is captured by a spectrometer as a unique spectral signature and is reported in relative intensity units (nm). Any change in the number of molecules bound to the biosensor causes a shift in the interference pattern that is measured in real time. This wavelength shift is a direct measure of the change in optical thickness (nm) of the biological layer.

Biosensor Types

For a list of all available biosensor types, please see here. Note that our facility has select sensor types (please check with us to confirm the specifics) available for purchase to users.


NU Core ID: BLItz Bio-Layer Interferometer