The Keck Biophysics Facility provides access to several analytical instruments for sample characterization.
Differential Scanning Calorimeter
The MicroCal (GE Healthcare) differential scanning calorimeter directly measures the enthalpy of a solution as a function of temperature.
Isothermal Titration Calorimeter
The MicroCal (GE Healthcare) ITC200 isothermal titration calorimeter is a powerful tool for label-free analysis of macromolecular interactions in solution.
This Agilent 1100 series high pressure liquid chromatography system is equipped for high sensitivity sample analysis.
The SEC-MALS-QELS system is composed of an Agilent 1260 series HPLC for size exclusion chromatography followed by a Wyatt DAWN HELEOS II multi-angle static light scattering detector, a Wyatt QELS dynamic light scattering detector, and a Wyatt T-rEx differential refractive index detector.
Dynamic Light Scattering
This batch mode DLS features a Brookhaven BI-200 goniometer and BI-9000 high speed correlator and a 3 watt argon-ion laser operating at 514 nm.
Analytical ultracentrifugation is a powerful biophysical technique for characterizing samples in terms of purity and composition, determining molecular weight, detecting complexes and their stoichiometries, and measuring equilibrium constants for self- and hetero-associating systems. The Beckman XLA -70 instrument measures absorbance in the UV-Vis range.
Surface Plasmon Resonance
The Reichert SR7500DC is a dual channel surface plasmon resonance spectrometer for label-free measurement of macromolecular association and dissociation.
The NanoSight LM10-HS is a unique instrument employing nanoparticle tracking analysis to characterize particle size distributions.
BLItz Bio-layer Interferometer
The BLItz is a micro volume instrument for characterizing the kinetics of macromolecular interactions using bio-layer interferometry with low cost disposable sensor probes. The instrument is located in the Ward building on the Chicago campus.