The Perkin Elmer LAMBDA 1050 is a high performance UV/Vis/NIR double beam, double monochromator, ratio-recording spectrophotometer. A 150 mm integrating sphere accessory allows diffuse reflectance and transmission measurements.
Circular Dichroism Spectrophotometer
Our state of the art Jasco J-815 CD instrument allows the simultaneous acquisition of fluorescence and CD data. Such experiments are particularly useful in assessing the extent to which protein unfolding and folding transitions are two-state in character.
Fluorescence Plate Reader
The Molecular Devices Gemini EM Fluorescence/Chemiluminescence Plate Reader complements the facility’s fluorimeters PC1 and K2 by accommodating high throughput fluorescence experiments.
The PanVera (Invitrogen) Beacon 2000 Variable Temperature Fluorescence Polarization System provides a simple, rapid, and highly sensitive way to study equilibrium binding for a range of biomolecules. It measures binding affinities directly in solution and can be used to study protein-protein, protein-DNA, or receptor-ligand binding and interactions.
Photon-Counting Fluorescence Spectrometer
The ISS PC1 spectrofluorimeter is a workstation for a wide variety of highly sensitive fluorescence studies. The PC1 has excitation/emission monochromators and optional polarizers suitable for fluorescence polarization/anisotropy and ratio measurements using both T- and L- configuration.
UV/Vis DAD Spectrophotometer
The HP (Agilent) 8452 diode array spectrophotometer is a basic analytical instrument for measuring UV/Vis spectra of solution samples in seconds.
NanoDrop 3300 Fluorimeter
The Thermo Scientific NanoDrop 3300 fluorimeter acquires full range visible emission spectra from micro-volume samples in seconds.
The Thermo Scientific NanoDroop 2000c acquires full range UV/Vis absorbance spectra from micro-volume or cuvette samples in seconds.
Differential Scanning Calorimeter
The MicroCal (GE Healthcare) differential scanning calorimeter directly measures the enthalpy of a solution as a function of temperature.
Isothermal Titration Calorimeter
The MicroCal (GE Healthcare) ITC200 isothermal titration calorimeter is a powerful tool for label-free analysis of macromolecular interactions in solution.
This Agilent 1100 series high pressure liquid chromatography system is equipped for high sensitivity sample analysis.
The SEC-MALS-QELS system is composed of an Agilent 1260 series HPLC for size exclusion chromatography followed by a Wyatt DAWN HELEOS II multi-angle static light scattering detector, a Wyatt QELS dynamic light scattering detector, and a Wyatt T-rEx differential refractive index detector.
Dynamic Light Scattering
This batch mode DLS features a Brookhaven BI-200 goniometer and BI-9000 high speed correlator and a 3 watt argon-ion laser operating at 514 nm.
Surface Plasmon Resonance
The Reichert SR7500DC is a dual channel surface plasmon resonance spectrometer for label-free measurement of macromolecular association and dissociation.
The NanoSight LM10-HS is a unique instrument employing nanoparticle tracking analysis to characterize particle size distributions.
BLItz Bio-layer Interferometer
The BLItz is a micro volume instrument for characterizing the kinetics of macromolecular interactions using bio-layer interferometry with low cost disposable sensor probes. The instrument is located in the Ward building on the Chicago campus.
Chemiluminescence & IR Imager
The Syngene PXi system offers fully automated capture and comprehensive analysis of chemiluminescent and fluorescent blots and gels.
LAS 4010 Gel Imaging System
The GE Healthcare ImageQuant LAS 4010 gel imaging system allows visualization, imaging and quantitative measurements of macromolecules in stained gels.
The Molecular Dynamics (GE Healthcare) Storm 860 phosphoimager images storage phosphor screens after exposure to radioisotope-labeled samples or electron scattering.
The GE Healthcare Typhoon 9400 allows sensitive detection and quantitation of fluorescent macromolecules in gel sandwiches, agarose and polyacrylamide gels, membranes, and microplates.
Real Time PCR
The Bio-Rad iCycler collects and reports PCR amplification and denaturation data in real-time.